Review



microarray laser scanner scanarray5000  (Packard Instruments)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Packard Instruments microarray laser scanner scanarray5000
    Microarray Laser Scanner Scanarray5000, supplied by Packard Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/scanner+scanarray5000/scanarray+4000/pm15331511-62-27-31
    Average 90 stars, based on 1 article reviews
    microarray laser scanner scanarray5000 - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Microarray:

    Article Title: Oligonucleotide microarray-based mutation detection of the K-ras gene in colorectal cancers with use of competitive DNA hybridization.
    Article Snippet: The PCR-amplified patient samples (above) were dissolved in 5 L of hybridization buffer (HybIt; TeleChem) and hybridized with the K-ras oligonucleotide microarray at 56 °C for 2.5 h in a general hybridization chamber (FINEPCR). .. The microarray was then rinsed with a buffer solution containing 2 g/L sodium dodecyl sulfate in 0.5 standard saline citrate, after which it was scanned with a microarray laser scanner (ScanArray5000; Packard Instruments) set to monitor the wavelengths 632.8, 543.8, and 594 nm, which correspond to Cy5, Cy3, and Alexa 594, respectively (9 ). .. The intensity of each spot, representing the amount of hybridized tumor DNA, was calculated by the Scanarray and Quantarray (Packard Instrument) image analysis software packages.

    Saline:

    Article Title: Oligonucleotide microarray-based mutation detection of the K-ras gene in colorectal cancers with use of competitive DNA hybridization.
    Article Snippet: The PCR-amplified patient samples (above) were dissolved in 5 L of hybridization buffer (HybIt; TeleChem) and hybridized with the K-ras oligonucleotide microarray at 56 °C for 2.5 h in a general hybridization chamber (FINEPCR). .. The microarray was then rinsed with a buffer solution containing 2 g/L sodium dodecyl sulfate in 0.5 standard saline citrate, after which it was scanned with a microarray laser scanner (ScanArray5000; Packard Instruments) set to monitor the wavelengths 632.8, 543.8, and 594 nm, which correspond to Cy5, Cy3, and Alexa 594, respectively (9 ). .. The intensity of each spot, representing the amount of hybridized tumor DNA, was calculated by the Scanarray and Quantarray (Packard Instrument) image analysis software packages.



    Similar Products

    two-channel confocal microarray scanner scanarray5000  (Lumonics Inc)
    90
    Lumonics Inc two-channel confocal microarray scanner scanarray5000
    Transcriptional Misregulation Resulting from DET1 Downregulation. The stages of fruit development at which the analysis were performed are indicated by MG (mature green), BR (breaker), and RR (red ripe). (A) Boxplots for log2 ratio (transgenic versus T56) of <t>microarray</t> expression data. P119 is shown in red and TFM7 in blue. Boxes show center quartiles (middle 50% of the data, whiskers extend to the most extreme data points that are no more than 1.5 times the interquartile range). The outliers are shown as filled circles. (B) Frequency distribution of expression values for P119 (red) and TFM7 (blue) across all developmental and ripening stages. The x axis represents log2 expression ratio of the DET1 variety versus T56 (control), indicating the relative changes in transcripts occurring within the data sets. The y axis shows the percentage of transcripts with a given relative expression value. An overwhelming proportion of expression values are above the T56 control level for P119, but TFM7 shows significant downregulation. (C) and (D) SOMs for TFM7 (C) and P119 (D). In each partition, the pattern reflects a general trend of expression gradient of the group across three developmental stages with vertical bars showing the variance in the group at each stage. A gene is assigned to a single partition with similar groups placed in nearby partitions. (E) and (F) Heat maps of the gene expression data for TFM7 (E) and P119 (F). Each horizontal line represents the gene expression across the three developmental stages. For each gene, log2 expression ratios of DET1 variety versus T56 are normalized across the three stages. Red indicates upregulation and blue downregulation with respect to the T56 background. The z-scores have been used to indicate the deviation from normal distribution (the distribution standard derivation) and calculated from the variable's value minus the population's mean divided by the sd of the population. The vertical color bars next to gene trees indicate genes belonging to SOM classes in (C) and (D).
    Two Channel Confocal Microarray Scanner Scanarray5000, supplied by Lumonics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/scanner+scanarray5000/scanarray+4000/pmc02879742-1164-7-13
    Average 90 stars, based on 1 article reviews
    two-channel confocal microarray scanner scanarray5000 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    scanner scanarray5000  (Lumonics Inc)
    90
    Lumonics Inc scanner scanarray5000
    Transcriptional Misregulation Resulting from DET1 Downregulation. The stages of fruit development at which the analysis were performed are indicated by MG (mature green), BR (breaker), and RR (red ripe). (A) Boxplots for log2 ratio (transgenic versus T56) of <t>microarray</t> expression data. P119 is shown in red and TFM7 in blue. Boxes show center quartiles (middle 50% of the data, whiskers extend to the most extreme data points that are no more than 1.5 times the interquartile range). The outliers are shown as filled circles. (B) Frequency distribution of expression values for P119 (red) and TFM7 (blue) across all developmental and ripening stages. The x axis represents log2 expression ratio of the DET1 variety versus T56 (control), indicating the relative changes in transcripts occurring within the data sets. The y axis shows the percentage of transcripts with a given relative expression value. An overwhelming proportion of expression values are above the T56 control level for P119, but TFM7 shows significant downregulation. (C) and (D) SOMs for TFM7 (C) and P119 (D). In each partition, the pattern reflects a general trend of expression gradient of the group across three developmental stages with vertical bars showing the variance in the group at each stage. A gene is assigned to a single partition with similar groups placed in nearby partitions. (E) and (F) Heat maps of the gene expression data for TFM7 (E) and P119 (F). Each horizontal line represents the gene expression across the three developmental stages. For each gene, log2 expression ratios of DET1 variety versus T56 are normalized across the three stages. Red indicates upregulation and blue downregulation with respect to the T56 background. The z-scores have been used to indicate the deviation from normal distribution (the distribution standard derivation) and calculated from the variable's value minus the population's mean divided by the sd of the population. The vertical color bars next to gene trees indicate genes belonging to SOM classes in (C) and (D).
    Scanner Scanarray5000, supplied by Lumonics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/scanner+scanarray5000/scanarray+4000/pm18800884-57-17-20
    Average 90 stars, based on 1 article reviews
    scanner scanarray5000 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    scanarray5000 two-channel laser confocal microarray scanner  (Lumonics Inc)
    90
    Lumonics Inc scanarray5000 two-channel laser confocal microarray scanner
    Transcriptional Misregulation Resulting from DET1 Downregulation. The stages of fruit development at which the analysis were performed are indicated by MG (mature green), BR (breaker), and RR (red ripe). (A) Boxplots for log2 ratio (transgenic versus T56) of <t>microarray</t> expression data. P119 is shown in red and TFM7 in blue. Boxes show center quartiles (middle 50% of the data, whiskers extend to the most extreme data points that are no more than 1.5 times the interquartile range). The outliers are shown as filled circles. (B) Frequency distribution of expression values for P119 (red) and TFM7 (blue) across all developmental and ripening stages. The x axis represents log2 expression ratio of the DET1 variety versus T56 (control), indicating the relative changes in transcripts occurring within the data sets. The y axis shows the percentage of transcripts with a given relative expression value. An overwhelming proportion of expression values are above the T56 control level for P119, but TFM7 shows significant downregulation. (C) and (D) SOMs for TFM7 (C) and P119 (D). In each partition, the pattern reflects a general trend of expression gradient of the group across three developmental stages with vertical bars showing the variance in the group at each stage. A gene is assigned to a single partition with similar groups placed in nearby partitions. (E) and (F) Heat maps of the gene expression data for TFM7 (E) and P119 (F). Each horizontal line represents the gene expression across the three developmental stages. For each gene, log2 expression ratios of DET1 variety versus T56 are normalized across the three stages. Red indicates upregulation and blue downregulation with respect to the T56 background. The z-scores have been used to indicate the deviation from normal distribution (the distribution standard derivation) and calculated from the variable's value minus the population's mean divided by the sd of the population. The vertical color bars next to gene trees indicate genes belonging to SOM classes in (C) and (D).
    Scanarray5000 Two Channel Laser Confocal Microarray Scanner, supplied by Lumonics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/scanner+scanarray5000/scanarray+4000/10__1094_slash_mpmi___19___1167-253-28-35
    Average 90 stars, based on 1 article reviews
    scanarray5000 two-channel laser confocal microarray scanner - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    scanarray5000 two-channel confocal microarray scanner  (Lumonics Inc)
    90
    Lumonics Inc scanarray5000 two-channel confocal microarray scanner
    Transcriptional Misregulation Resulting from DET1 Downregulation. The stages of fruit development at which the analysis were performed are indicated by MG (mature green), BR (breaker), and RR (red ripe). (A) Boxplots for log2 ratio (transgenic versus T56) of <t>microarray</t> expression data. P119 is shown in red and TFM7 in blue. Boxes show center quartiles (middle 50% of the data, whiskers extend to the most extreme data points that are no more than 1.5 times the interquartile range). The outliers are shown as filled circles. (B) Frequency distribution of expression values for P119 (red) and TFM7 (blue) across all developmental and ripening stages. The x axis represents log2 expression ratio of the DET1 variety versus T56 (control), indicating the relative changes in transcripts occurring within the data sets. The y axis shows the percentage of transcripts with a given relative expression value. An overwhelming proportion of expression values are above the T56 control level for P119, but TFM7 shows significant downregulation. (C) and (D) SOMs for TFM7 (C) and P119 (D). In each partition, the pattern reflects a general trend of expression gradient of the group across three developmental stages with vertical bars showing the variance in the group at each stage. A gene is assigned to a single partition with similar groups placed in nearby partitions. (E) and (F) Heat maps of the gene expression data for TFM7 (E) and P119 (F). Each horizontal line represents the gene expression across the three developmental stages. For each gene, log2 expression ratios of DET1 variety versus T56 are normalized across the three stages. Red indicates upregulation and blue downregulation with respect to the T56 background. The z-scores have been used to indicate the deviation from normal distribution (the distribution standard derivation) and calculated from the variable's value minus the population's mean divided by the sd of the population. The vertical color bars next to gene trees indicate genes belonging to SOM classes in (C) and (D).
    Scanarray5000 Two Channel Confocal Microarray Scanner, supplied by Lumonics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/scanner+scanarray5000/scanarray+4000/pm16167902-320-12-18
    Average 90 stars, based on 1 article reviews
    scanarray5000 two-channel confocal microarray scanner - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    microarray laser scanner scanarray5000  (Packard Instruments)
    90
    Packard Instruments microarray laser scanner scanarray5000
    Transcriptional Misregulation Resulting from DET1 Downregulation. The stages of fruit development at which the analysis were performed are indicated by MG (mature green), BR (breaker), and RR (red ripe). (A) Boxplots for log2 ratio (transgenic versus T56) of <t>microarray</t> expression data. P119 is shown in red and TFM7 in blue. Boxes show center quartiles (middle 50% of the data, whiskers extend to the most extreme data points that are no more than 1.5 times the interquartile range). The outliers are shown as filled circles. (B) Frequency distribution of expression values for P119 (red) and TFM7 (blue) across all developmental and ripening stages. The x axis represents log2 expression ratio of the DET1 variety versus T56 (control), indicating the relative changes in transcripts occurring within the data sets. The y axis shows the percentage of transcripts with a given relative expression value. An overwhelming proportion of expression values are above the T56 control level for P119, but TFM7 shows significant downregulation. (C) and (D) SOMs for TFM7 (C) and P119 (D). In each partition, the pattern reflects a general trend of expression gradient of the group across three developmental stages with vertical bars showing the variance in the group at each stage. A gene is assigned to a single partition with similar groups placed in nearby partitions. (E) and (F) Heat maps of the gene expression data for TFM7 (E) and P119 (F). Each horizontal line represents the gene expression across the three developmental stages. For each gene, log2 expression ratios of DET1 variety versus T56 are normalized across the three stages. Red indicates upregulation and blue downregulation with respect to the T56 background. The z-scores have been used to indicate the deviation from normal distribution (the distribution standard derivation) and calculated from the variable's value minus the population's mean divided by the sd of the population. The vertical color bars next to gene trees indicate genes belonging to SOM classes in (C) and (D).
    Microarray Laser Scanner Scanarray5000, supplied by Packard Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/scanner+scanarray5000/scanarray+4000/pm15331511-62-27-31
    Average 90 stars, based on 1 article reviews
    microarray laser scanner scanarray5000 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Transcriptional Misregulation Resulting from DET1 Downregulation. The stages of fruit development at which the analysis were performed are indicated by MG (mature green), BR (breaker), and RR (red ripe). (A) Boxplots for log2 ratio (transgenic versus T56) of microarray expression data. P119 is shown in red and TFM7 in blue. Boxes show center quartiles (middle 50% of the data, whiskers extend to the most extreme data points that are no more than 1.5 times the interquartile range). The outliers are shown as filled circles. (B) Frequency distribution of expression values for P119 (red) and TFM7 (blue) across all developmental and ripening stages. The x axis represents log2 expression ratio of the DET1 variety versus T56 (control), indicating the relative changes in transcripts occurring within the data sets. The y axis shows the percentage of transcripts with a given relative expression value. An overwhelming proportion of expression values are above the T56 control level for P119, but TFM7 shows significant downregulation. (C) and (D) SOMs for TFM7 (C) and P119 (D). In each partition, the pattern reflects a general trend of expression gradient of the group across three developmental stages with vertical bars showing the variance in the group at each stage. A gene is assigned to a single partition with similar groups placed in nearby partitions. (E) and (F) Heat maps of the gene expression data for TFM7 (E) and P119 (F). Each horizontal line represents the gene expression across the three developmental stages. For each gene, log2 expression ratios of DET1 variety versus T56 are normalized across the three stages. Red indicates upregulation and blue downregulation with respect to the T56 background. The z-scores have been used to indicate the deviation from normal distribution (the distribution standard derivation) and calculated from the variable's value minus the population's mean divided by the sd of the population. The vertical color bars next to gene trees indicate genes belonging to SOM classes in (C) and (D).

    Journal: The Plant Cell

    Article Title: Integrative Transcript and Metabolite Analysis of Nutritionally Enhanced DE-ETIOLATED1 Downregulated Tomato Fruit [W]

    doi: 10.1105/tpc.110.073866

    Figure Lengend Snippet: Transcriptional Misregulation Resulting from DET1 Downregulation. The stages of fruit development at which the analysis were performed are indicated by MG (mature green), BR (breaker), and RR (red ripe). (A) Boxplots for log2 ratio (transgenic versus T56) of microarray expression data. P119 is shown in red and TFM7 in blue. Boxes show center quartiles (middle 50% of the data, whiskers extend to the most extreme data points that are no more than 1.5 times the interquartile range). The outliers are shown as filled circles. (B) Frequency distribution of expression values for P119 (red) and TFM7 (blue) across all developmental and ripening stages. The x axis represents log2 expression ratio of the DET1 variety versus T56 (control), indicating the relative changes in transcripts occurring within the data sets. The y axis shows the percentage of transcripts with a given relative expression value. An overwhelming proportion of expression values are above the T56 control level for P119, but TFM7 shows significant downregulation. (C) and (D) SOMs for TFM7 (C) and P119 (D). In each partition, the pattern reflects a general trend of expression gradient of the group across three developmental stages with vertical bars showing the variance in the group at each stage. A gene is assigned to a single partition with similar groups placed in nearby partitions. (E) and (F) Heat maps of the gene expression data for TFM7 (E) and P119 (F). Each horizontal line represents the gene expression across the three developmental stages. For each gene, log2 expression ratios of DET1 variety versus T56 are normalized across the three stages. Red indicates upregulation and blue downregulation with respect to the T56 background. The z-scores have been used to indicate the deviation from normal distribution (the distribution standard derivation) and calculated from the variable's value minus the population's mean divided by the sd of the population. The vertical color bars next to gene trees indicate genes belonging to SOM classes in (C) and (D).

    Article Snippet: Scanning of arrays was performed on a two-channel confocal microarray scanner (ScanArray5000; GSI Lumonics) with ScanArray software v3.1 (Packard Biochip Technologies).

    Techniques: Transgenic Assay, Microarray, Expressing, Control, Gene Expression

    The Effect of DET1 Downregulation on Photorespiration-Related Gene Expression Levels in P119 Mature Green Fruit. (A) Pathways and processes involved in photorespiration; steps in the pathways where transcripts have been measured are numbered. (B) Changes in gene expression levels relative to levels determined in control (T56 background) samples. Data from microarray analysis have been used; the experimental design of these experiments is provided in Methods. The data are expressed as means ± sd. Transcripts are labeled as follows, 1, ribulose-1,5-bisphosphate carboxylase small chain 3b; 2, glycolate oxidase; 3, hydroxypyruvate reductase; 4, glycine hydroxymethyl transferase; 5, transketolase; 6, phosphoglycolate phosphatase; 7, photosystem II protein 16; 8, photosystem II 22-kD protein; 9, photosystem II 5-kD protein; 10, photosystem II psbY; 11, photosystem II reaction center; 12, photosystem I subunit II; 13, photosystem I subunit III; 14, photosystem I subunit VI; 15, photosystem I subunit X; 16, photosystem I subunit psaN. Pa, pheophytin; Pq, plastoquinone; Cyt, cytochrome bf complex; Pc, plastocyanin; Fd, ferrodoxin; 3-PGA, 3-phosphoglycerate; GA-3-P, glyceraldehyde-3-phosphate. Stoichiometries of Calvin cycle components shown in parentheses.

    Journal: The Plant Cell

    Article Title: Integrative Transcript and Metabolite Analysis of Nutritionally Enhanced DE-ETIOLATED1 Downregulated Tomato Fruit [W]

    doi: 10.1105/tpc.110.073866

    Figure Lengend Snippet: The Effect of DET1 Downregulation on Photorespiration-Related Gene Expression Levels in P119 Mature Green Fruit. (A) Pathways and processes involved in photorespiration; steps in the pathways where transcripts have been measured are numbered. (B) Changes in gene expression levels relative to levels determined in control (T56 background) samples. Data from microarray analysis have been used; the experimental design of these experiments is provided in Methods. The data are expressed as means ± sd. Transcripts are labeled as follows, 1, ribulose-1,5-bisphosphate carboxylase small chain 3b; 2, glycolate oxidase; 3, hydroxypyruvate reductase; 4, glycine hydroxymethyl transferase; 5, transketolase; 6, phosphoglycolate phosphatase; 7, photosystem II protein 16; 8, photosystem II 22-kD protein; 9, photosystem II 5-kD protein; 10, photosystem II psbY; 11, photosystem II reaction center; 12, photosystem I subunit II; 13, photosystem I subunit III; 14, photosystem I subunit VI; 15, photosystem I subunit X; 16, photosystem I subunit psaN. Pa, pheophytin; Pq, plastoquinone; Cyt, cytochrome bf complex; Pc, plastocyanin; Fd, ferrodoxin; 3-PGA, 3-phosphoglycerate; GA-3-P, glyceraldehyde-3-phosphate. Stoichiometries of Calvin cycle components shown in parentheses.

    Article Snippet: Scanning of arrays was performed on a two-channel confocal microarray scanner (ScanArray5000; GSI Lumonics) with ScanArray software v3.1 (Packard Biochip Technologies).

    Techniques: Gene Expression, Control, Microarray, Labeling